Methyl-CpG-binding protein 2 (MeCP2) is a chromatin regulator whose loss of function causes Rett syndrome. It has been unclear how the gene-expression changes caused by loss of MeCP2 relate to the protein’s DNA-binding sites. New work uses the ‘CUT&RUN’ technique to identify DNA-binding sites that are largely devoid of methylation — a modification known to recruit MeCP2 to DNA. This is a preview of subscription content, access via your institution Access options Access through your institution Change institution Buy or subscribe Access Nature and 54 other Nature Portfolio journals Get Nature+, our best-value online-access subscription $29.99 / 30 days cancel any time Learn more Subscribe to this journal Receive 12 print issues and online access $209.00 per year only $17.42 per issue Learn more Buy this article Purchase on SpringerLink Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout Additional access options: Log in Learn about institutional subscriptions Read our FAQs Contact customer support Fig. 1: MeCP2 binds to unmethylated DNA. ReferencesAmir, R. E. et al. Nat. Genet. 23, 185–188 (1999).Article CAS PubMed Google Scholar Skene, P. J. & Henikoff, S. eLife 6, e21856 (2017).Article PubMed PubMed Central Google Scholar Mishra, G. P. et al. Nat. Neurosci. https://doi.org/10.1038/s41593-024-01808-y (2024).Article PubMed Google Scholar Lewis, J. D. et al. Cell 69, 905–914 (1992).Article CAS PubMed Google Scholar Chen, L. et al. Proc. Natl Acad. Sci. USA 112, 5509–5514 (2015).Article CAS PubMed PubMed Central Google Scholar Lagger, S. et al. PLoS Genet. 13, e1006793 (2017).Article PubMed PubMed Central Google Scholar Gabel, H. W. et al. Nature 522, 89–93 (2015).Article CAS PubMed PubMed Central Google Scholar Lister, R. et al. Science 341, 1237905 (2013).Article PubMed PubMed Central Google Scholar Lavery, L. A. et al. eLife 9, e52981 (2020).Article CAS PubMed PubMed Central Google Scholar Tillotson, R. et al. Mol. Cell 81, 1260–1275 (2021).Article CAS PubMed PubMed Central Google Scholar Skene, P. J. et al. Mol. Cell 37, 457–468 (2010).Article CAS PubMed PubMed Central Google Scholar Stroud, H. et al. Cell 171, 1151–1164.e1116 (2017).Article CAS PubMed PubMed Central Google Scholar Boxer, L. D. et al. Mol. Cell 77, 294–309.e299 (2020).Article CAS PubMed Google Scholar Liu, Y. et al. Neuron 112, 1943–1958.e10 (2024).Article CAS PubMed Google Scholar Download referencesAuthor informationAuthors and AffiliationsDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USAJun Young Sonn & Huda Y. ZoghbiJan and Dan Duncan Neurological Research Institute, Texas Children’s Hospital, Houston, TX, USAJun Young Sonn & Huda Y. ZoghbiDepartment of Pediatrics, Baylor College of Medicine, Houston, TX, USAHuda Y. ZoghbiDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, USAHuda Y. ZoghbiDepartment of Neurology, Baylor College of Medicine, Houston, TX, USAHuda Y. ZoghbiHoward Hughes Medical Institute, Baylor College of Medicine, Houston, TX, USAHuda Y. ZoghbiAuthorsJun Young SonnView author publicationsYou can also search for this author in PubMed Google ScholarHuda Y. ZoghbiView author publicationsYou can also search for this author in PubMed Google ScholarCorresponding authorCorrespondence to Huda Y. Zoghbi.Ethics declarations Competing interests The authors declare no competing interests. Rights and permissionsReprints and permissionsAbout this articleCite this articleSonn, J.Y., Zoghbi, H.Y. MeCP2 goes into unmethylated territories. Nat Neurosci (2024). https://doi.org/10.1038/s41593-024-01846-6Download citationPublished: 17 December 2024DOI: https://doi.org/10.1038/s41593-024-01846-6Share this articleAnyone you share the following link with will be able to read this content:Get shareable linkSorry, a shareable link is not currently available for this article.Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative

MeCP2 goes into unmethylated territories

Methyl-CpG-binding protein 2 (MeCP2) is a chromatin regulator whose loss of function causes Rett syndrome. It has been unclear how the gene-expression changes caused by loss of MeCP2 relate to the protein’s DNA-binding sites. New work uses the ‘CUT&RUN’ technique to identify DNA-binding sites that are largely devoid of methylation — a modification known to […]

Methyl-CpG-binding protein 2 (MeCP2) is a chromatin regulator whose loss of function causes Rett syndrome. It has been unclear how the gene-expression changes caused by loss of MeCP2 relate to the protein’s DNA-binding sites. New work uses the ‘CUT&RUN’ technique to identify DNA-binding sites that are largely devoid of methylation — a modification known to recruit MeCP2 to DNA.

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**Fig. 1: MeCP2 binds to unmethylated DNA.**

References

Amir, R. E. et al. Nat. Genet. 23, 185–188 (1999).

Article
CAS
PubMed

Google Scholar
Skene, P. J. & Henikoff, S. eLife 6, e21856 (2017).

Article
PubMed
PubMed Central

Google Scholar
Mishra, G. P. et al. Nat. Neurosci. https://doi.org/10.1038/s41593-024-01808-y (2024).

Article
PubMed

Google Scholar
Lewis, J. D. et al. Cell 69, 905–914 (1992).

Article
CAS
PubMed

Google Scholar
Chen, L. et al. Proc. Natl Acad. Sci. USA 112, 5509–5514 (2015).

Article
CAS
PubMed
PubMed Central

Google Scholar
Lagger, S. et al. PLoS Genet. 13, e1006793 (2017).

Article
PubMed
PubMed Central

Google Scholar
Gabel, H. W. et al. Nature 522, 89–93 (2015).

Article
CAS
PubMed
PubMed Central

Google Scholar
Lister, R. et al. Science 341, 1237905 (2013).

Article
PubMed
PubMed Central

Google Scholar
Lavery, L. A. et al. eLife 9, e52981 (2020).

Article
CAS
PubMed
PubMed Central

Google Scholar
Tillotson, R. et al. Mol. Cell 81, 1260–1275 (2021).

Article
CAS
PubMed
PubMed Central

Google Scholar
Skene, P. J. et al. Mol. Cell 37, 457–468 (2010).

Article
CAS
PubMed
PubMed Central

Google Scholar
Stroud, H. et al. Cell 171, 1151–1164.e1116 (2017).

Article
CAS
PubMed
PubMed Central

Google Scholar
Boxer, L. D. et al. Mol. Cell 77, 294–309.e299 (2020).

Article
CAS
PubMed

Google Scholar
Liu, Y. et al. Neuron 112, 1943–1958.e10 (2024).

Article
CAS
PubMed

Google Scholar

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Author information

Authors and Affiliations

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA

Jun Young Sonn & Huda Y. Zoghbi
Jan and Dan Duncan Neurological Research Institute, Texas Children’s Hospital, Houston, TX, USA

Jun Young Sonn & Huda Y. Zoghbi
Department of Pediatrics, Baylor College of Medicine, Houston, TX, USA

Huda Y. Zoghbi
Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA

Huda Y. Zoghbi
Department of Neurology, Baylor College of Medicine, Houston, TX, USA

Huda Y. Zoghbi
Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX, USA

Huda Y. Zoghbi